The Resolute®️ AutoPak application was designed in direct reaction to this marketplace need to have for packing regularity by absolutely automated operations.
Join Sartorius as we explore tips on how to transfer a standalone batch mAb chromatography system into a linked DSP.
An ineffective HPLC column can impact your Evaluation, leading to inaccurate outcomes, which means more time spent on exactly the same challenge.
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Be a part of our webinar to understand what tends to make monoliths ideal for the troubles of purifying significant biomolecules.
Physiochemical properties on the cell phase applied and interaction with the analyte and stationary phases
Limit of quantitation – the lessen or higher quantity of the analyte which may be reliably quantified
Big biomolecules are becoming progressively popular within the biotherapeutic industry. Ground breaking alternatives - which include monolithic chromatography - are necessary to purify these new modalities.
And In case you have any more queries regarding tips on how to best look after your column, run an analysis, or anything pertaining to operate, Phenomenex provides a cost-free technological company by Reside Chat, which allows you to speak with Technological Gurus 24/seven around the globe. They can easily assist with Just about here any dilemma quickly.
The Sartorius household of CIM® monoliths is perfect for preparatory and analytical applications in addition to immobilization screening. Pre-configured merchandise packs are readily available, a combination of columns in addition to a protocol to fit unique demands.
The unique selectivity of combined-method monolithic columns makes certain the right purification of the most difficult substantial biomolecules.
Molecular size and form: Larger sized or irregularly shaped molecules demand a wider pore size from the stationary stage.
Void volume is the more info amount of Place within a column that is occupied by solvent. It is the space inside the column that may be beyond the column's interior packing content. Void quantity is measured over a chromatogram as the very first part peak detected, which is generally the solvent which was existing within the sample mixture; Preferably the sample solvent flows throughout the column with out interacting Together with the column, but is still detectable as distinct with the HPLC solvent. The void quantity is made use of as being a correction issue.
Preparative LC separations slide underneath three groups: Analytical, semi-preparative and preparative and the aim from the separation establishes the scale, column size and movement fee.